FastQCFastQC Report
Sat 7 Apr 2018
zr2096_6_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_6_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24237290
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAC351.020453E-982.4069061
CAGTGCT8750.079.581531
GCCTGCT22550.078.204341
CTGTGCT15650.076.088171
CCGTGCT11150.075.755231
GCCTGGG2450.073.998041
GTGTGCT16400.071.603571
GGGTGCT10150.069.822611
GCCTGGT5150.068.805771
CCCTGCT27450.068.7474751
CCAGGCT3700.067.930021
GCCTGGA2550.067.864511
CCTGGCT4450.066.666271
CGCTGCT7100.066.157651
CCCTGGG3550.064.9969941
GTCTGCT8150.064.712171
GCCTAGG3200.064.3803941
CCCTGGA3700.063.4755941
TGTGCTC56450.063.465752
GCCGGCT650.063.389931
Produced by FastQC (version 0.11.5)