FastQCFastQC Report
Sat 7 Apr 2018
zr2096_5_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_5_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31778715
Sequences flagged as poor quality0
Sequence length20-100
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT9300.075.539151
CAGTGCT3950.072.85491
CTGTGCT6600.072.457191
CCGTGCT5550.070.152281
CCTGGCT2850.065.3363341
CAGGGCT601.2732926E-1163.4801941
GGCCTAG601.2732926E-1163.4801941
GGCCCAA2000.061.3641851
TGTGCTC32150.061.175572
AGTGCTC14900.061.032092
GCCTGGG1250.060.9409871
CCAGGCT2450.060.457331
GTGCTCT76050.059.0647853
GTCTGCT4300.058.067151
GCCTAGG1900.057.9117551
GGCCAAC1850.057.1893651
GGCCTAT2900.056.913281
CCCTGCT13500.056.4268381
GCCGGCT1350.056.4268381
CTGGCTC11700.056.3955462
Produced by FastQC (version 0.11.5)