FastQCFastQC Report
Sat 7 Apr 2018
zr2096_3_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_3_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29892002
Sequences flagged as poor quality0
Sequence length20-100
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAG302.82962E-884.285351
CCTGGCT8750.081.395561
CCAGGCT7000.080.0710751
CAGTGCT6900.080.011
GCCTGCT20300.078.680161
CCGTGCT9500.076.7440261
TGCAGCT6150.076.062381
GCCTGAT9200.075.581971
CTGTGCT8050.075.385651
ACTGGCT7400.075.1734161
GTCAGCT7300.075.048591
GCCTGGG3400.074.369431
GCCTGGT6050.073.8367541
CATGGCT5000.072.48541
CTAGGCT6550.070.773961
GCCTGGA3050.070.468081
GTGCTAC302.7860533E-670.2377851
GTCTGCT7050.069.93891
GGCAGCT2850.069.498441
CCCTGGG6200.069.331491
Produced by FastQC (version 0.11.5)