FastQCFastQC Report
Sat 7 Apr 2018
zr2096_2_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_2_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30798582
Sequences flagged as poor quality0
Sequence length20-100
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT35600.075.47541
CAGTGCT13400.075.269041
CTGTGCT21300.075.006131
CCGTGCT18250.072.509031
GTCTGCT12750.070.562811
CCTGGCT5500.066.751321
CCCTGCT50850.066.6452941
CGCTGCT12550.066.222361
GTGTGCT24050.065.4230351
GCCTGGG3300.064.795241
GCCTGGT5500.064.550731
GGGCACC251.1847208E-464.550731
GCCTGAT5500.063.8171921
GCATGCT2750.063.0836641
CCAGGCT5250.063.013811
TGTGCTC81300.062.9849132
ACTGGCT5300.061.6581231
GGGTGCT13350.060.742961
GGGCCAA402.8072282E-760.5163081
GGCCTAT2050.059.04031
Produced by FastQC (version 0.11.5)