FastQCFastQC Report
Sat 7 Apr 2018
zr2096_10_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_10_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17717127
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT5450.079.15021
CCAGGCT2850.077.9265751
GCCAGCT3650.076.058471
GCTGGCT2250.074.030251
TGCAGCT2550.070.345491
CCTGGCT3250.069.649761
GGCCCGA501.6734703E-1068.335621
CTGTGCT2850.067.436461
GCCTGAT3250.067.021471
CTAGGCT2500.066.627231
CCGTGCT2200.066.005991
GGCAGCT1600.064.0646441
GGCCATC601.2732926E-1164.0646441
CAGTGCT2300.063.1361661
ACTGGCT2800.062.539291
CAGGCTC8800.062.123812
TGTGCTC8700.061.3651122
ACAGGCT1750.061.013941
GGCTGCT3300.060.829051
CGCTGCT2600.060.779271
Produced by FastQC (version 0.11.5)