FastQCFastQC Report
Wed 14 Mar 2018
9_31_S31_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename9_31_S31_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595446
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA21480.3607380014308602Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG2750.069.6970656
GGTCGCC2750.069.66765655
ATCTCGG2850.069.4624647
TAGATCT2950.069.42158544
CCTGGTG2800.069.3690837
TCCTGGT2650.069.3632536
GTCCTGG2650.069.3632535
CGTCCTG2700.069.3632434
CTCGGTG2850.068.2840549
TCTCGGT2900.068.2820848
AGATCTC2900.068.2361445
GCCGTAT2900.067.4075459
GAAGAGC3050.067.0777746
GAGCGTC3050.067.0777749
CTGGTGT3000.065.9006238
CGCCGTA3000.063.94836458
GATCTCG3100.063.8499146
TGGTCGC3000.063.8135454
TCGCCGT3050.062.86815357
GTACGTC3050.062.5353831
Produced by FastQC (version 0.11.5)