FastQCFastQC Report
Wed 14 Mar 2018
8_30_S30_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8_30_S30_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665233
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA12360.18579956195799066Illumina Single End PCR Primer 1 (96% over 32bp)
TGGAATAAATTAGTTGATTTTTGGGGTATGATTTTGATGTAAAAGGGTTA7840.11785344383095847No Hit
TGGTGTATTTTTGTTGTTTTTTGAAAATTTGAGGGAGATATTGTGATTTTTGTGTTAGGTTGTATTTATATTTGT7140.10733081491748003No Hit
TGGATTTTTTAAATTAGATAAGATAATATTATTATATTTTTATAGGGGGT6910.10387337970305141No Hit
TGGTTGTTTTGTTTAGTTTTTTGAAGTGGAGATGGAGTAGGGAAGGGGTAAGTGAGGGTTTTGGTGAATTTTTTG6740.10131788410977809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC900.069.343769
CGGAGTG150.002309152469.343761
CGGGGTA301.0926124E-769.343761
CGGGGAA150.002309152469.343761
AAGAGCG900.069.343767
TGGAGTC353.9981387E-969.343765
GAAGAGC900.069.343766
CGGGTAG208.3046405E-569.343761
CGGAAGG150.002309152469.343761
CGGAAAT208.3046405E-569.343761
CGGGAGT208.3046405E-569.343761
AGCGTCG900.069.3437610
CGGTATG150.002309152469.343761
CGGTACG150.002309152469.343761
GATCGGA1000.065.876571
TCGTGTA1000.065.8765714
CGTCGTG1000.065.8765712
CGTATCA850.065.8040161
GCGTCGT950.065.6940911
GTCGCCG850.065.6100356
Produced by FastQC (version 0.11.5)