FastQCFastQC Report
Wed 14 Mar 2018
7_29_S29_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename7_29_S29_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612994
Sequences flagged as poor quality0
Sequence length35-76
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATAAATTAGTTGATTTTTGGGGTATGATTTTGATGTAAAAGGGTTA10580.17259549033106358No Hit
TGGATTTTTTAAATTAGATAAGATAATATTATTATATTTTTATAGGGGGT8260.13474846409589653No Hit
TGGTGTATTTTTGTTGTTTTTTGAAAATTTGAGGGAGATATTGTGATTTTTGTGTTAGGTTGTATTTATATTTGT6580.10734199682215487No Hit
TGGTTGTTTTGTTTAGTTTTTTGAAGTGGAGATGGAGTAGGGAAGGGGTAAGTGAGGGTTTTGGTGAATTTTTTG6190.10097978120503627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTG207.8660523E-490.94507670
GAGCGTC600.069.307049
CGGAGAA150.002313921569.307041
CGGGGGT301.0964868E-769.307041
TGGAGTC208.325935E-569.307045
CGGATGG301.0964868E-769.307041
CGGATAC150.002313921569.307041
TCGTGAT208.325935E-569.3070410
CGGAATT401.4733814E-1069.307041
CGGAAAA150.002313921569.307041
AGTCGTG208.325935E-569.307048
CGGTGTG301.0964868E-769.307041
CGGTGTA208.325935E-569.307041
GCGTCGT600.069.3070411
CGTCGTG600.069.3070412
CGGTCGT150.002313921569.307041
CGGTACG150.002313921569.307041
CGGAAGA650.069.307034
ATCGGAA650.069.307032
AAGAGCG700.064.356537
Produced by FastQC (version 0.11.5)