FastQCFastQC Report
Wed 14 Mar 2018
7_29_S29_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename7_29_S29_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612994
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATAAATTAGTTGATTTTTGGGGTATGATTTTGATGTAAAAGGGTTA7940.12952818461518384No Hit
TGGATTTTTTAAATTAGATAAGATAATATTATTATATTTTTATAGGGGGT6210.10130604867258082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA650.069.31319
AGAGCAC650.069.31318
CGGAGAG301.0964504E-769.307441
ACGTCTG650.069.3074415
GATACGA253.0121191E-669.307443
CTCCAGT600.069.3074424
CGGATAC253.0121191E-669.307441
CGGGTAA150.002313867769.307441
CGGAAGT150.002313867769.307441
CGGGAGA150.002313867769.307441
GCGTTAA150.002313867769.3074414
CGGTATA800.069.307441
CGGTAGT150.002313867769.307441
CGTCTGA650.069.3074416
AAGAGCA700.064.362177
GAAGAGC700.064.362176
AGCACAC700.064.3621710
TCTGAAC700.064.3569118
GCACACG700.064.3569111
CTTCTGC650.064.31721556
Produced by FastQC (version 0.11.5)