FastQCFastQC Report
Wed 14 Mar 2018
6_36_S36_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6_36_S36_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31352
Sequences flagged as poor quality0
Sequence length35-76
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA1930.6155907119163052Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAA1100.35085480990048484Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.456968E-1269.59360562
GCCGTAT455.456968E-1269.59360559
CCGGATC150.002256152769.593660
CGGATCA150.002256152769.593661
CCGTATC401.3460522E-1069.593660
GCCGGAT208.0372025E-569.593659
CGTATCA500.069.593661
CGCCGTA401.3460522E-1069.593658
CGCCGGA301.03285856E-769.593658
GTAGATC1200.069.4824343
ATCCCGG150.002270536569.4824347
GTTGATC150.002270536569.4824343
GAGCGTC1800.069.482439
TGGTCGC750.069.4824354
CCGTGTA1550.069.4824339
GTGTTCA1850.069.4824329
ATCTCGG950.069.4824347
ATCTTGG208.101294E-569.4824347
GGCGCCG150.002270536569.4824356
TCGGTTG150.002270536569.4824350
Produced by FastQC (version 0.11.5)