FastQCFastQC Report
Wed 14 Mar 2018
6_36_S36_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6_36_S36_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31352
Sequences flagged as poor quality0
Sequence length35-76
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13534.315514161775963TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2500.7973972952283745TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTT1850.069.585658
TGCTTGA1900.069.585660
GCTTGAA1900.069.585661
CTGCTTG1900.069.585659
TGAAAAA1900.069.585664
ACACGTC1900.069.4744413
GTATGCC1900.069.4744447
AATCTCG1850.069.4744441
ATCTCGT1850.069.4744442
CAGTCAC1900.069.4744427
GTCACAT1900.069.4744429
CACACGT1900.069.4744412
GAAATCT1850.069.4744439
ACGTCTG1900.069.4744415
TGCCGTC1900.069.4744450
CCAGTCA1900.069.4744426
CACGTCT1900.069.4744414
TATGCCG1900.069.4744448
AAGAGCA1900.069.474447
CTCCAGT1850.069.4744424
Produced by FastQC (version 0.11.5)