FastQCFastQC Report
Wed 14 Mar 2018
5_35_S35_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_35_S35_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21862
Sequences flagged as poor quality0
Sequence length35-76
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA2120.9697191473790138Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC252.8418326E-669.606954
TCTCGGT252.8418326E-669.606948
GATCTCG252.8418326E-669.606946
CGGTGGT252.8418326E-669.606951
AGATCTC252.8418326E-669.606945
TAGATCT252.8418326E-669.606944
GGTCGCC252.8418326E-669.606955
GTAGATC301.01670594E-769.60689543
ATCTCGG301.01670594E-769.60689547
GTCGCCG301.01670594E-769.60689556
TCGGTGG207.97468E-569.60689550
GTATCAT301.01670594E-769.60689562
CCGTATC301.01670594E-769.60689560
TCGCCGT301.01670594E-769.60689557
CGTATCA301.01670594E-769.60689561
GCCGTAT301.01670594E-769.60689559
GGGTGTA301.01670594E-769.60689539
CGCCGTA301.01670594E-769.60689558
GAGCGTC353.7180143E-969.447259
GATCGGA353.7180143E-969.447251
Produced by FastQC (version 0.11.5)