FastQCFastQC Report
Wed 14 Mar 2018
5_35_S35_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_35_S35_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21862
Sequences flagged as poor quality0
Sequence length35-76
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2371.084072820418992TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT450.20583661147196047TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA353.7180143E-969.447251
ACACGTC401.0682925E-860.76634213
ACGTCTG401.0682925E-860.76634215
CACGTCT401.0682925E-860.76634214
AAGAGCA401.0682925E-860.7663427
CTCCAGT401.0682925E-860.76634224
ACTCCAG401.0682925E-860.76634223
GTCTGAA401.0682925E-860.76634217
GAAGAGC401.0682925E-860.7663426
TCGGAAG401.0682925E-860.7663423
GAGCACA401.0682925E-860.7663429
CGGAAGA401.0682925E-860.7663424
CTGAACT401.0682925E-860.76634219
AGAGCAC401.0682925E-860.7663428
GAACTCC401.0682925E-860.76634221
ATCGGAA401.0682925E-860.7663422
TCTGAAC401.0682925E-860.76634218
GGAAGAG401.0682925E-860.7663425
AGCACAC401.0682925E-860.76634210
TGAACTC401.0682925E-860.76634220
Produced by FastQC (version 0.11.5)