FastQCFastQC Report
Wed 14 Mar 2018
4_28_S28_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_28_S28_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279065
Sequences flagged as poor quality0
Sequence length35-76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA24480.8772149857560066Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAA10240.3669396018848655Illumina Single End PCR Primer 1 (96% over 32bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9460.3389891243975418No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTCGGTGGGCGCCGTATCATTAAAAAA2990.107143497034741Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT150.002090869271.09035562
ACGTACT150.002253925269.7573351
GATCGGA13150.067.79541
AAGAGCG13050.067.523627
GCGTCGT12900.067.24145511
CCGTGTA11150.067.19540439
GTAGGGA13000.066.9889818
CGCCGTA3900.066.81251558
TCGGAAG13300.066.771993
GAGCGTC13100.066.740389
AGCCGTG11850.066.6863837
AGCGTCG13200.066.4955410
TCTCGGT6800.066.4258548
ATCTCGG7750.066.3052247
ATCGGAA13450.066.283242
TAGATCT8050.066.2659844
AGAGCGT13250.066.2446068
AGATCTC7800.066.229345
CGTCGTG13050.066.20479612
CGGAAGA13450.066.027324
Produced by FastQC (version 0.11.5)