FastQCFastQC Report
Wed 14 Mar 2018
4_28_S28_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_28_S28_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279065
Sequences flagged as poor quality0
Sequence length35-76
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA106673.8224069661190048TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT19500.6987619371830935TruSeq Adapter, Index 7 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9580.3432891978571301No Hit
CGGCGACCACCGAGATCTACACGGCTCTGAACACTCTTTCCCTACACGAC3450.1236271119631627Illumina RNA PCR Primer (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA13100.068.0334241
TCGGAAG13150.067.513063
GCCGTCT13050.067.3434651
TGCCGTC13050.067.306750
CGGAAGA13200.067.2573244
ACGTCTG13200.067.25732415
GTATGCC13150.067.0687247
CACACGT13300.067.0103612
ATCGGAA13300.067.010362
TGAACTC13150.066.989720
GAACTCC13100.066.9827121
ATGCCGT13150.066.9381349
CTGCTTG13150.066.82651559
TGCTTGA13300.066.788160
ACACGTC13350.066.75938413
CGTATGC13150.066.7562746
AGTCACG13100.066.7200328
CCGTCTT13100.066.6510752
CTCGTAT13050.066.6153944
TATGCCG13250.066.6108448
Produced by FastQC (version 0.11.5)