FastQCFastQC Report
Wed 14 Mar 2018
3_27_S27_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_27_S27_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231652
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA172367.440471051404693Illumina Single End PCR Primer 1 (97% over 34bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13480.5819073437742821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT20500.068.40330559
CCGTATC20400.068.21110560
CGCCGTA20700.067.9023458
CGTATCA20300.067.8233161
GGTCGCC21150.067.8125255
GATCGGA22500.067.770341
TCGCCGT20850.067.6473557
TCGGAAG22600.067.622433
CGTGTAG22350.067.6105315
TCGTGTA22250.067.605714
GTAGGGA22150.067.4457718
GTATCAT20400.067.40729562
ATCGGAA22750.067.176572
GTCGCCG21450.067.0882356
AGCGTCG22850.067.03287510
GAGCGTC22850.067.0328759
GCGTCGT22750.067.0256111
CTCGGTG21200.066.9970349
CGTCGTG22750.066.8746612
GTCGTGT22650.066.8666513
Produced by FastQC (version 0.11.5)