FastQCFastQC Report
Wed 14 Mar 2018
3_27_S27_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_27_S27_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231652
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA163047.038143422029595TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT29061.2544679087596913TruSeq Adapter, Index 7 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13680.5905409838896276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA22600.068.0629261
GATCGGA22700.067.922251
TCGGAAG22800.067.624353
CTGCTTG22800.067.2878759
CTCGTAT22650.067.12533644
CCGTCTT22600.067.0900252
CGGAAGA23000.067.036314
ACGTCTG22850.067.0255315
TGCTTGA22900.067.01980660
GCCGTCT22650.066.9628951
CGTCTTC22650.066.9208653
AGAGCAC23100.066.894768
ACACGTC23000.066.8870113
GTATGCC22850.066.786847
GAAGAGC23150.066.750286
ATCGGAA23100.066.746112
GGAAGAG23200.066.606425
CACACGT23100.066.5974512
CACGTCT23050.066.5929414
TATGCCG22950.066.56904648
Produced by FastQC (version 0.11.5)