FastQCFastQC Report
Wed 14 Mar 2018
2_26_S26_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_26_S26_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168954
Sequences flagged as poor quality0
Sequence length35-76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA72104.267433739361009Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA8700.5149330587023687Illumina Single End PCR Primer 1 (96% over 32bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4650.27522284172023154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA8850.068.0881958
GCCGTAT9000.067.9632859
GTAGGGA10100.067.84556618
TCGGAAG10100.067.504633
CCGTATC8950.067.260260
TAGGGAA10150.067.17210419
CGTGTAG10150.067.17210415
CGTATCA9000.066.9477761
GTCGTGT10250.066.8527113
TCGCCGT8900.066.792257
TCGTGTA10300.066.52817514
CGGAAGA10200.066.505234
GATCGGA10200.066.505231
AGCGTCG10100.066.48183410
AAGAGCG10000.066.457987
GTCGCCG9200.066.34502456
TGGTCGC9100.066.1831954
GTAGATC9750.066.16057643
GTGCCTC8250.065.985831
AGTGTGC9450.065.953328
Produced by FastQC (version 0.11.5)