FastQCFastQC Report
Wed 14 Mar 2018
2_26_S26_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_26_S26_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168954
Sequences flagged as poor quality0
Sequence length35-76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA78194.627886880452667TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT14190.8398735750559324TruSeq Adapter, Index 7 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4790.2835091208257869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA9950.068.3216660
ACACGTC9950.068.1686313
TCTGCTT10000.068.002558
AGCACAC10000.067.8277910
GAGCACA10100.067.4971169
TCGGAAG10050.067.490333
AGAGCAC10050.067.490338
GAGATTC10050.067.26736534
GTCTTCT10150.067.2633854
CACACGT10100.067.1562312
CACGTCT10100.067.1562314
AGTCACG10050.067.1477428
GCCGTCT10100.067.0476551
CTCGTAT10200.067.01424444
AGATTCC10150.066.9643335
CGTCTTC10150.066.8781353
GATCGGA10200.066.835391
GCACACG10150.066.8254111
ACGTCTG10150.066.8254115
GAAGAGC10100.066.815336
Produced by FastQC (version 0.11.5)