FastQCFastQC Report
Wed 14 Mar 2018
20_8_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20_8_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314922
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGA150.002252182269.7732158
TCGCCGG150.002262207769.6951357
GTCGCCG650.069.6617156
GGTCGCC650.069.62833455
GTGTCAG1100.069.2742731
TGTCAGT1100.069.2742732
GTCAGTA1100.069.2742733
GAAGAGC1100.069.263276
CAGTACG1150.066.2834135
TGTGTCA1150.066.25181630
CTCGGTG900.065.5820249
ATCTCGG850.065.35509547
TCTCGGT850.065.35509548
AGATCTC850.065.282345
CGCCGTA600.063.9587758
TCAGTAC1200.063.5014234
GCCGTAT550.063.47082559
GAGCGTC1150.063.240379
TAGATCT900.061.64570644
AGAGCGT1250.060.9516758
Produced by FastQC (version 0.11.5)