FastQCFastQC Report
Wed 14 Mar 2018
20_8_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20_8_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314922
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7150.22704034649849805TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1100.070.037161
CTGCTTG1100.069.8463859
TCTGCTT1100.069.8016758
TTCTGCT1100.069.7235357
CTTCTGC1100.069.6901156
TGCCGTC1100.069.50127450
GTATGCC1100.069.46805647
TATGCCG1100.069.46805648
ATGCCGT1100.069.46805649
ACGTCTG1100.069.28042615
ACTCCAG1050.069.28042623
GTCTGAA1100.069.28042617
GAGCACA1100.069.2804269
CTGAACT1100.069.28042619
AGAGCAC1100.069.2804268
AGCACAC1100.069.28042610
TGAACTC1050.069.28042620
GCACACG1100.069.28042611
AGTCACA1050.069.28042628
CGTCTGA1100.069.28042616
Produced by FastQC (version 0.11.5)