FastQCFastQC Report
Wed 14 Mar 2018
1_25_S25_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_25_S25_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488137
Sequences flagged as poor quality0
Sequence length35-76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA337526.9144522951548435TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT59701.2230173086653953TruSeq Adapter, Index 7 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11520.23599931986307124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA45200.068.014331
AGAGCAC45450.067.792048
TCGGAAG45300.067.788013
GAGCACA45550.067.643219
ATCGGAA45500.067.641712
ACACGTC45500.067.4900413
CGGAAGA45450.067.488374
ACGTCTG45500.067.41421515
CACACGT45600.067.3420412
CACGTCT45600.067.3420414
GCACACG45550.067.3402211
CGTCTGA45600.067.2663716
AGTCACG44750.067.2333228
GAAGAGC45700.067.194696
TCTCGTA43500.067.1858943
AAGAGCA45850.067.125367
AGCACAC45750.067.1212510
GTCTGAA45600.067.1150417
TGCTTGA44400.067.10210460
CTGAACT45600.066.96371519
Produced by FastQC (version 0.11.5)