FastQCFastQC Report
Wed 14 Mar 2018
19_7_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename19_7_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371667
Sequences flagged as poor quality0
Sequence length35-76
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA8130.21874419843569648Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT650.069.7172859
CGCCGTA650.069.6795958
TCGCCGT650.069.6513657
GTCGCCG700.069.58555656
GGTCGCC700.069.55739655
CTCGGTG750.069.463749
TCTCGGT750.069.44498448
ATCTCGG750.069.4169447
GAGCGTC750.069.379589
AAGAGCG750.069.379587
GAAGAGC750.069.379586
GTCCTGG750.069.3795835
GCGTCGT750.069.3795811
CGTCGTG750.069.3795812
CTGGTGT700.069.3795738
CGTCCTG800.069.3795734
TCGGTGG800.065.1309850
GATCTCG800.065.0783846
AGATCTC800.065.0696145
TAGATCT800.065.0696144
Produced by FastQC (version 0.11.5)