FastQCFastQC Report
Wed 14 Mar 2018
19_7_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename19_7_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371667
Sequences flagged as poor quality0
Sequence length35-76
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8570.2305827528405804TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA750.069.900662
GCTTGAA750.069.82484461
TGCTTGA750.069.73038560
CTGCTTG750.069.6926759
TCTGCTT750.069.65558
TTCTGCT750.069.6267757
CTTCTGC750.069.56156
TCTTCTG750.069.5328555
GTCTTCT750.069.5047254
GCCGTCT750.069.4578951
TGCCGTC750.069.4485350
TATGCCG750.069.4204748
GTATGCC750.069.3924347
CGTATGC750.069.3924346
ACACGTC700.069.3550913
CAGTCAC750.069.3550927
GTCACAT750.069.3550929
ACGTCTG700.069.3550915
CCAGTCA750.069.3550926
CTCCAGT750.069.3550924
Produced by FastQC (version 0.11.5)