FastQCFastQC Report
Wed 14 Mar 2018
18_10_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename18_10_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525421
Sequences flagged as poor quality0
Sequence length35-76
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA10600.20174298324581622Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1400.067.5288258
TGGTCGC1600.067.5160754
CGTATCA1250.067.49541561
CTCGGTG1600.067.2387349
GTGCCTC1450.066.7910331
TCGGAAG2050.065.79573
AGCGTCG1900.065.5293210
GCCGTAT1500.065.43690559
AGATCTC1600.064.9642345
CCGTATC1300.064.8241660
GCCTCTA1550.064.7134333
GATCGGA2000.063.9821051
GAGCGTC2000.063.9821059
AGAGCGT1950.063.8490838
CGTGTAG1850.063.56147415
GGTCGCC1650.063.41901855
GGTGGTC1700.063.41678652
TCTCGGT1650.063.07382648
ATCTCGG1650.063.04974447
GTATCAT1400.062.82946862
Produced by FastQC (version 0.11.5)