FastQCFastQC Report
Wed 14 Mar 2018
18_10_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename18_10_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525421
Sequences flagged as poor quality0
Sequence length35-76
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15890.30242415129962447TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2050.067.4661561
GTCACTC2050.067.46615629
CCGGAGA2000.067.4368435
TCCGGAG2000.067.4304134
AGTCACT2000.067.42399628
TATGCCG2000.065.8959448
TCGGAAG2100.065.859823
GAATCTC2050.065.8359240
AGAATCT2050.065.8233739
GCACACG2050.065.779511
ACGTCTG2050.065.779515
GAGCACA2050.065.77959
TCTCGTA2100.064.29903443
GTATGCC2050.064.26417547
CACTCCG2100.064.2194431
ACTCCGG2100.064.2194432
GAACTCC2100.064.21332621
CGTCTGA2100.064.21332616
CACACGT2100.064.21332612
CACGTCT2100.064.21332614
Produced by FastQC (version 0.11.5)