FastQCFastQC Report
Wed 14 Mar 2018
17_9_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename17_9_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403861
Sequences flagged as poor quality0
Sequence length35-76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA99712.468918761653143TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT18340.45411663914069444TruSeq Adapter, Index 6 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9440.2337437880855047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14500.065.9760148
AGAGCAC14550.065.654318
GATCGGA14500.065.636441
CGTCTTC14500.065.59531453
TCGGAAG14600.065.421363
CCGTCTT14600.065.2453252
GTATGCC14600.065.2118547
GCCGTCT14650.065.1294251
CGTATGC14600.065.0807146
CCGGAGA14300.065.0238335
TGCTTGA14600.064.9008160
GCACACG14700.064.7434211
CTCGTAT14650.064.6473244
CTCCGGA14700.064.5826633
CACTCCG14750.064.5399731
TCCGGAG14600.064.3607334
ACTCCGG14800.064.35387432
CGGAAGA14900.064.341894
GTCACTC14850.064.31998429
ATCGGAA14850.064.3199842
Produced by FastQC (version 0.11.5)