FastQCFastQC Report
Wed 14 Mar 2018
16_40_S40_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16_40_S40_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771805
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA11630.15068573020387274Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAG150.002269910969.6451859
GGCCGTA301.06241714E-769.62707558
GGCCGGG252.9327712E-669.6225657
GGTCTCC208.152555E-569.6044755
TGGTCTC208.1683014E-569.57735454
CAGGACG150.002296351169.4421134
GCAGTAC208.250047E-569.4376133
TGTGGCA252.9806215E-669.4331129
GCCGTAT2150.068.0255259
GTATCAT1800.065.8401862
TCGCCGT2200.064.8755757
GGTCGCC3550.064.7027455
TGGTCGC3300.064.3063554
GGTCATT550.063.3755862
CTCGGTG4300.063.0395649
TCTCGGT4250.062.9594148
GTCGCCG3650.062.93824856
CGCCGTA2300.062.05891458
CGCCGGA1700.059.38780258
CGGGTCA650.058.94585860
Produced by FastQC (version 0.11.5)