FastQCFastQC Report
Wed 14 Mar 2018
16_40_S40_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16_40_S40_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771805
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA53930.6987516276779756TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA14590.18903738638645773TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT10070.13047337086440228TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCT150.002281258369.5576653
CGGCTTC150.002283020469.5441352
CCGGCTT150.002284195769.535151
GCCGGCT150.002286547869.5170650
TGCCGGC150.002288313169.5035349
ATGCCGG150.002288901769.4990248
GATCGGA7350.062.818971
CAGAAAT9200.048.69328337
TGCTTGA9400.048.54083660
CTGCTTG9400.048.5282259
GCTTGAA9450.048.29342761
GAGCACA9850.048.2848439
AGAGCAC9850.048.2848438
GCCGTCT9450.048.1962951
TTCTGCT9350.048.02727557
ACGTCTG9850.047.93239615
AGCACAC9850.047.93239610
GCACACG9850.047.93239611
TGCCGTC9500.047.93018350
ATGCCGT9500.047.92085649
Produced by FastQC (version 0.11.5)