FastQCFastQC Report
Wed 14 Mar 2018
15_39_S39_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15_39_S39_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671325
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA7520.11201727926116263Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1100.066.4480459
AGATCTC1050.066.178845
CTGGTGT1050.066.1590838
GGTCGCC1000.066.1020155
CTCGGTG1000.066.04288549
ATCTCGG1000.066.023247
TCCTGGT1000.065.9936936
CCTGGTG1000.065.9936937
GTCCTGG950.065.81087535
CGTCCTG950.065.81087534
GAGCGTC1200.063.678129
TAGATCT1100.063.1659744
TCTCGGT1000.062.55761748
GTCGCCG1150.060.5097956
GAAGAGC1300.058.7798046
TGGTCGC1150.054.4466254
CGCCGTA1300.053.53587758
TCGCCGT1300.053.53188357
AGTGTAC1300.053.43618428
GGTGGTC1200.052.16244552
Produced by FastQC (version 0.11.5)