FastQCFastQC Report
Wed 14 Mar 2018
15_39_S39_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15_39_S39_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671325
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9120.13585074293375043TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC1200.069.4668210
AAGAGCA1250.069.466817
GAGCACA1250.069.466819
AGAGCAC1250.069.466818
GCCGTCT1250.066.7577651
CTTGAAA1200.066.74637662
TGCCGTC1250.066.7428350
ATGCCGT1250.066.73785449
GCTTGAA1200.066.7314361
TATGCCG1250.066.72790548
GTATGCC1250.066.7179647
TCTGCTT1200.066.6965758
TTCTGCT1200.066.691657
CAGTCAC1250.066.6881427
CCAGTCA1250.066.6881426
CTCCAGT1250.066.6881424
ACTCCAG1250.066.6881423
CATTCAG1250.066.6881433
TCCAGTC1250.066.6881425
GCACACG1250.066.6881411
Produced by FastQC (version 0.11.5)