FastQCFastQC Report
Wed 14 Mar 2018
14_34_S34_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename14_34_S34_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691626
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA35460.512704843369104Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4650.069.9016659
GGTCGCC4850.069.6676955
TGGTCGC4850.069.6372854
CGGTCGC150.00227082969.63727654
CTATGTG5000.069.2945637
CCTATGT1050.069.2895536
CCCTATG1000.069.2845435
CTCTATG3700.069.2845435
GCCTCTA3500.069.27952633
TGCCCCT1000.069.2745132
TGCCGCT208.346065E-569.2745132
GTGCCCC1000.069.2745131
TGCCTCT3500.069.27450632
TGTGCCC1000.069.269530
TGTTCCT150.002319023569.2694930
GTGTTCC150.002319689169.2644929
GTGTGCC4700.069.2644929
CTCGGTG4750.068.75423449
ATCTCGG4950.068.7339147
AGATCTC5000.068.6911145
Produced by FastQC (version 0.11.5)