FastQCFastQC Report
Wed 14 Mar 2018
14_34_S34_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename14_34_S34_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691626
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA47450.6860644336679073TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT9060.13099565372036331TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC152.4221971E-4122.29510570
CTCCAGT5250.069.2825824
TCCAGTC5250.069.2825825
CCAGTCA5250.069.2825826
AGTCACA5300.069.2825728
GTCTGAA5450.069.2825717
TGAACTC5400.069.2825720
CGTCTGA5400.069.2825716
CTGAACT5450.069.2825719
GAGCACA5450.069.282579
AGAGCAC5450.069.282578
TCTGAAC5500.068.65272518
AAGAGCA5500.068.6527257
AGCACAC5450.068.6469510
ACGTCTG5450.068.6469515
ACTCCAG5350.068.6350823
CTGCTTG5350.068.61800459
TCTGCTT5350.068.5579458
TGCCGTC5400.068.2365150
GCTTGAA5350.068.1087261
Produced by FastQC (version 0.11.5)