13_33_S33_L001_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	13_33_S33_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645468
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	24

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA	8061	1.248861291342096	Illumina Single End PCR Primer 1 (97% over 34bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGCCG	20	7.978677E-5	69.90629	56
GGCCGCC	15	0.002244649	69.84063	55
TGGCCGC	20	8.037682E-5	69.802376	54
GTGGCCG	15	0.0022565194	69.74781	53
GGTCGCC	900	0.0	67.90061	55
GAAGAGC	1000	0.0	67.796364	6
GTAGGCT	960	0.0	67.743965	31
ATCTCGG	925	0.0	67.619576	47
TCTCGGT	910	0.0	67.604416	48
AGATCTC	930	0.0	67.587555	45
TAGGCTA	965	0.0	67.39297	32
CTCGGTG	915	0.0	67.276924	49
GATCTCG	940	0.0	67.26416	46
TCGCCGT	910	0.0	67.25975	57
GTCGCCG	915	0.0	67.23228	56
CTATAGT	975	0.0	67.129425	36
TGGTCGC	905	0.0	67.102844	54
GCTATAG	970	0.0	67.08199	35
GGCTATA	970	0.0	67.07679	34
TGTAGGC	975	0.0	67.05655	30