FastQCFastQC Report
Wed 14 Mar 2018
13_33_S33_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename13_33_S33_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645468
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA80871.252889376390464TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT15340.23765701785371235TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG9950.069.0459559
TTCTGCT10050.068.9156757
TCAGAAA10000.068.9129836
GCTTGAA10000.068.80884661
TGCTTGA10000.068.73852560
TCTGCTT10050.068.6429758
CAGAAAT9750.068.5648937
TGCCGTC9950.068.55041550
CTTCTGC10100.068.53152556
CTTGAAA10050.068.53123562
ATTCAGA9950.068.5206534
ATGCCGT9950.068.5130149
AGCACAC10100.068.499110
AGTCACA10100.068.499128
AAGAGCA10100.068.49917
AGAGCAC10100.068.49918
CATTCAG9900.068.4958833
TATGCCG10000.068.4755448
TCTTCTG10100.068.4671355
GTATGCC10050.068.4647247
Produced by FastQC (version 0.11.5)