FastQCFastQC Report
Wed 14 Mar 2018
12_38_S38_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12_38_S38_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865269
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG500.069.5327649
TCTCGGT500.069.5166848
AGATCTC500.069.5126645
TAGATCT550.069.5046244
ATCTCGG550.063.1933347
GGTCGCC501.4551915E-1162.5939655
GAAGAGC700.059.558186
GCCGTAT601.8189894E-1257.9741459
GTCGCCG553.8198777E-1156.90360356
TCGCCGT608.913048E-1152.1676757
GAGCGTC800.052.1134079
TGGTCGC751.6370905E-1146.36053554
CGCCGTA904.794856E-934.77844658
GTATCAT1154.1836756E-1133.2836762
GATCTCG1055.9299055E-1033.10126546
AGCGTCG1305.456968E-1232.06978610
GGTGGTC1202.4701876E-928.9736652
AAGAGCG1452.0008883E-1128.7522267
CCCACGT500.00747422527.7938164
GTAGATC1509.586074E-1025.48355743
Produced by FastQC (version 0.11.5)