FastQCFastQC Report
Wed 14 Mar 2018
12_38_S38_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12_38_S38_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865269
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA600.069.592761
TGCTTGA600.069.5765960
CTGCTTG600.069.5725659
TTCTGCT600.069.56048657
TCTGCTT600.069.56048658
CTTCTGC600.069.5524456
TGCCGTC600.069.54037550
ATGCCGT600.069.5363549
TATGCCG600.069.5202648
GTATGCC550.069.5162547
TCAGAAA650.069.4881336
CAGAAAT650.069.4881337
TTCAGAA650.069.4881335
AAGAGCA600.069.488127
GAGCACA600.069.488129
AGAGCAC600.069.488128
ATTCAGA600.069.4841134
CAGTCAC600.069.48009527
CCAGTCA650.069.48009526
CTCCAGT600.069.48009524
Produced by FastQC (version 0.11.5)