FastQCFastQC Report
Wed 14 Mar 2018
11_37_S37_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename11_37_S37_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695562
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG600.063.67719715
AAGAGCA600.063.6771977
GTCTGAA600.063.67719717
GAGCACA600.063.6771979
CTGAACT600.063.67719719
AGAGCAC600.063.6771978
AGCACAC600.063.67719710
TGAACTC600.063.67719720
CGTCTGA600.063.67719716
CTTGAAA650.058.9272162
GCTTGAA650.058.91871661
TGCTTGA650.058.9144760
TCTGCTT650.058.8890258
TTCTGCT650.058.88053557
TCTTCTG650.058.8678255
ATGCCGT650.058.82546649
GTATGCC650.058.81700547
CAGAAAT650.058.787437
TTCAGAA650.058.787435
ATTCAGA650.058.787434
Produced by FastQC (version 0.11.5)