FastQCFastQC Report
Wed 14 Mar 2018
10_32_S32_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10_32_S32_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574346
Sequences flagged as poor quality0
Sequence length35-76
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA21000.36563325939416313Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAA6980.12152953097958373Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGA208.025913E-569.8217158
GCCGTCT353.785317E-969.8217159
GCGCCGT401.4006218E-1069.7665657
GGGCCGG150.002253988869.7665657
TTGCCGT401.4006218E-1069.7665657
GGGGCCG150.002259485469.7237356
GGGGGCC150.002263417769.6931755
CTTGGTG208.180713E-569.5529349
TCTTGGT252.9538787E-669.53467648
ATCTTGG252.9600233E-669.5103647
GATCTTG252.964638E-669.49213446
AGATCTT301.0776057E-769.4799845
TAGAACT150.002295060369.4496443
TCGCCGG1100.066.5953657
GCCGTAT3200.066.5488259
GGTCGCC4350.066.48888455
GTATCAT2800.064.98855662
CTCGGTG5100.064.7796949
TCTCGGT5300.064.2867848
TAGATCT5850.064.1185644
Produced by FastQC (version 0.11.5)