FastQCFastQC Report
Wed 25 Nov 2015
D4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53285111
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA5342991.0027172506030813No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA2730030.5123438703167945No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA2657630.49875658511812054No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT2558450.4801435057534177No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT2207880.4143521442603357No Hit
CTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAG1443370.27087679333163067No Hit
TGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTT1336150.2507548496990088No Hit
GTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCT1163050.21826922721433387No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1144550.21479733804063952No Hit
CTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTAAATTTTT1078380.20237923497991775No Hit
ATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCC1020740.19156195433279666No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA1016440.19075497468701905No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA969640.18197203342599774No Hit
GTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGAAACCAA959790.18012348702811184No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA882460.1656109902820696No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTTTTGG848500.15923772777727724No Hit
GTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAA818230.15355696641037306No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA813880.15274060328034222No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT781410.14664696860629606No Hit
TTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTA770010.1445075341965601No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC758340.1423174289718567No Hit
CCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCC665280.12485288807974895No Hit
TTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGAA663000.12442500119780177No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC662100.12425609848124367No Hit
AATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAA643020.12067536089021191No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA639960.12010109165391436No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG563660.1057818946834886No Hit
CTTCACCTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTT559910.10507813336449652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAAA1651400.041.1884775
TTGTGAA1744450.038.707954
CTCCCGT59500.038.400861
GTGAAAA1867500.036.5062336
CGGGGCA18150.036.1925431
CGCGCAC20200.035.2963033
GAAAAGT2107800.032.040138
AAAAGTG1901200.031.8495259
CGGAGGG869400.031.0660721
CGCGCTG8000.030.9406911
GGAGGGT896000.030.6627852
TCGCGCA23400.030.26652
TTTGTGA2187600.029.7001193
GGGTTGC218650.029.5640095
TTTTGTG2250100.029.5069432
AGACGTG129150.029.1117954
CGTGCAT129700.028.9295567
CCGGAGG59700.028.7838941
CCCGTCA70850.026.9025763
GAGGGTT229150.026.59243
Produced by FastQC (version 0.11.2)