FastQCFastQC Report
Wed 25 Nov 2015
D3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70550834
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA2509190.3556570288028062No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1356040.19220750813519794No Hit
CTTCGATTACCTTCTTCATTAAGGTGCAAAGAATAACACCGTCCCTCAAA1217130.17251815903409448No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC1147270.16261607906718722No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT1071200.1518337827161618No Hit
CGGAGGGTTGCCTTGAGTTGCAACTTCAATTAATTTAATTTTAGTTTGAC1014330.1437729283256949No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA966480.13699058468961545No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA901150.12773059493527744No Hit
CTCGAGTCTGTCAGCCATTTGATCTAACTGGAAGGTCAGCTCCGCGCTTT887190.12575187984312136No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG881170.12489859439507123No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA846330.1199603111708077No Hit
GGGGACTCTCAGCTTACTTAAGAAAATTGTTCCATTCGTCTGAGTGCCGT778520.11034880182989758No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT712630.10100943668504331No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG711940.10091163486458572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTA380850.035.4269373
CCGGAGG138650.034.7443051
CGATTAC390150.034.4213834
CGGAGGG670500.032.082871
CGCGCAC38050.031.214373
GGAGGGT703700.030.1592432
CGCCTAT63000.029.8620911
TCGCGCA41500.029.7657812
CTTCGAT506200.029.3568951
GGGTTGC408100.028.996115
GAGGGTT425050.027.0038813
TGTGAAA1499700.026.9434745
GTTGCCT439600.026.6702567
CGGGGCA48350.026.6279891
GTGAAAA1529950.026.5282146
CTCCCGT92650.026.4075031
GCGAGGT200100.026.234981
GAAAAGT1587100.025.3351978
TTGTGAA1657100.024.6595344
CGCGTGG26300.024.386171
Produced by FastQC (version 0.11.2)