FastQCFastQC Report
Wed 25 Nov 2015
D3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70550834
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA2318260.32859427288981446No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1256210.17805742735798133No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC1200640.1701808372669273No Hit
CTTCGATTACCTTCTTCATTAAGGTGCAAAGAATAACACCGTCCCTCAAA1160780.16453101036339274No Hit
CGGAGGGTTGCCTTGAGTTGCAACTTCAATTAATTTAATTTTAGTTTGAC1105050.15663174158933402No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT1051170.14899469508751662No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA984420.1395334320215123No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG873940.1238738014067984No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA843950.11962296576111346No Hit
CTCGAGTCTGTCAGCCATTTGATCTAACTGGAAGGTCAGCTCCGCGCTTT839520.1189950497254221No Hit
GGGGACTCTCAGCTTACTTAAGAAAATTGTTCCATTCGTCTGAGTGCCGT827500.11729131366469744No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA822920.1166421363636892No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG723520.10255300454704759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAGG135500.038.889931
CGGAGGG712700.031.9998721
TCGATTA371050.031.3727553
GGGTTGC429950.031.1189775
CGATTAC391300.030.7208464
GGAGGGT748800.030.5780812
CGCGCAC38300.030.28223
TCGCGCA40550.029.8915712
CGCCTAT58950.029.5546721
GAGGGTT434850.029.3058053
GTTGCCT464850.028.6600157
TGTGAAA1474950.026.3216865
GCGAGGT198800.026.1477971
CTTCGAT486500.026.1152741
CGGGGCA46350.025.9836181
GTGAAAA1527600.025.6476786
CCGGGAG141450.025.206221
GAAAAGT1565600.024.721798
CTCGGGT64400.023.7272831
TTGTGAA1634250.023.6493744
Produced by FastQC (version 0.11.2)