FastQCFastQC Report
Wed 25 Nov 2015
D2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69624273
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA2973420.4270665777723812No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT1553480.22312333516214955No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1534700.22042599999571988No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT1531190.21992186546780892No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC1268910.1822510951029966No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1265430.18175126941720454No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1156390.16609006459571937No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA910380.1307561229400557No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA883650.12691694461211825No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA867150.12454708144672476No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA768010.11030779452447569No Hit
CACAGATTTGGGACAAAGCATTGCTTTAGCGAAAAAGTCCTTTCGTACAA750460.10778712188492079No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG749130.10759609655098303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT117100.042.1876371
TGGCCCG53300.039.2883878
CGCCTAT86350.035.997331
CCCGTCA132050.034.682863
ATCCTAG139200.031.9454612
GCCGCGT70600.030.276416-17
TGTGCGC30950.029.604697
GTGCGCG33250.027.7053038
GTGAAAA1722200.026.6942736
GATCCTA168500.026.5866411
AGACGTG158450.026.5386414
CGGGAGC127850.026.429452
TGTGAAA1743000.026.2313755
CCGGGAG140950.025.9585651
GAAAAGT1757500.025.8402718
CCCATCA761050.025.8049663
CTCCCAT763750.025.5501381
CGTGCAT160200.025.3673537
TCCCATC761050.024.94342
AAAATCG1070400.024.1250959
Produced by FastQC (version 0.11.2)