FastQCFastQC Report
Wed 25 Nov 2015
D2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69624273
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA2599900.37341862083069793No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1451960.2085422134318013No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT1424140.20454648050687724No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1336610.1919747154846414No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT1295880.18612474416788524No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC1291720.18552725139406484No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1186590.17042763232874258No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA856630.12303611414369814No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA847020.12165584838494473No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA780530.11210601796876213No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA771620.11082629185944966No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG710450.10204056277901817No Hit
CACAGATTTGGGACAAAGCATTGCTTTAGCGAAAAAGTCCTTTCGTACAA703950.10110698031992377No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCAC698180.1002782463523892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCCG49600.042.2280738
CTCCCGT106650.041.0044751
CGCCTAT85500.037.734641
CCCGTCA120200.034.680613
ATCCTAG140500.030.9553952
GCCGCGT70300.029.52367816-17
GTCGCGC5550.028.2942091
GCGCCGT9450.027.695391
TGTGCGC30050.027.3429877
AGACGTG152500.027.0228314
CCGGGAG134050.026.7303581
GATCCTA164800.026.5070931
GTGCGCG32050.026.2294758
GTGAAAA1640250.025.9703836
CGTGCAT154500.025.7915737
TGTGAAA1672600.025.4141335
CGGGAGC127000.025.3008172
GAAAAGT1703350.024.8967868
TCGAGTG62300.024.471547
CGCGCTG14650.023.3868371
Produced by FastQC (version 0.11.2)