FastQCFastQC Report
Wed 25 Nov 2015
D1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74550563
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA8330661.117450984240052No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT3339040.44788930701972035No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT2697380.36181886379583744No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG2221520.2979883599269398No Hit
TGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTT1965670.263669370276922No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCAC1578950.2117958518971882No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC1286000.17250037400790655No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA1279660.17164994448130458No Hit
CTTTAGTACCTTTTGCATAAGGGTTTTTCAAGATAAATTTAAGAATTTAA1205470.1616983093742699No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA1124350.15081710382254257No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1123290.15067491844427788No Hit
GTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAA1077820.14457570226532024No Hit
ATTTTTTTGAGTTAAGAAATCGTGGTAGAGATTTGATTAAACTAAAGAAT1037940.13922631275098485No Hit
CTTCAATTGAAAAGCAAATTATCGCGCTACCTTAGGACGCTCACGTTAAC1017060.13642552907346925No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA1010390.13553083428759619No Hit
CTTTCATACCATCCTTCAATTGAAAAGCAAATTATCGCGCTACCTTAGGA978850.13130014859847539No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA976980.1310493121292726No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG974580.13072738297093747No Hit
CTTCGTTAACCCTTTCATACCATCCTTCAATTGAAAAGCAAATTATCGCG970300.13015327597190648No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT966940.1297025751502373No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA962220.12906944780551155No Hit
CTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTAAATTTTT960040.1287770288200238No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCACC924710.1240379633350321No Hit
TTTCAAGATAAATTTAAGAATTTAATTTTCCCGAATAAAAGAGAGTTATT911050.12220564987550797No Hit
AGAAACCAAAGATTTTTTAAATATCAATTTCACCATTTTTCAGCTTAACC892410.11970533341243848No Hit
CTTCACCTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTT861530.11556317824185983No Hit
GAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTG832100.11161552193777531No Hit
ATAAAAACGAGTAAAAATATTATTTTCTTGGTATCACTGTTTTGTTTTAC824360.1105773004021445No Hit
ATTTATTTAAAAATAAGCCTATGATCGGAGGGTCAGTGATTATGATAAAA810950.10877852122994698No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAA810690.10874364557112734No Hit
GTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCACCATT801910.10756592140021799No Hit
GCACCTTCTTCGTTAACCCTTTCATACCATCCTTCAATTGAAAAGCAAAT786000.10543179935475472No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACC779310.10453442182589553No Hit
CACCATTTTTCAGCTTAACCCAAAAAATTTTACCCCTTAAATAGTTAAAG771420.10347607971786879No Hit
ATTGTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTA770470.1033486494260278No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA754400.10119306543667551No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA752210.1008993050796947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT106300.042.547111
CTCCCGT112050.042.100281
TGTGAAA2038700.041.541945
CCCGTCA110900.038.8596463
TTGTGAA2196250.038.709354
GTGAAAA2382850.035.703476
CGCGCTG10850.034.9921531
AAAAGTG2592850.032.722649
AGACGTG189300.032.694944
CGATTAC143400.031.4432954
GAAAAGT2672650.031.4349338
TCGAGTC226900.030.3291132
CGTGCAT199800.030.1057727
TTTTGTG3156850.027.9706082
TTTGTGA3130850.027.2626823
GTCAGGG416900.026.8310781
CCGTCAA168700.026.5026554
AAAATCG983300.025.0498059
CGAGTCT305250.024.9242653
CCCATCA734000.024.7719753
Produced by FastQC (version 0.11.2)