FastQCFastQC Report
Wed 25 Nov 2015
C0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66933289
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA4101740.6128101668513556No Hit
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA3629540.542262311359001No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT2968170.4434519869477802No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC2397860.3582462532208749No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA2260830.33777363009906775No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA2219760.331637669859612No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA2018970.30163914401397485No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT1859640.27783484537865755No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1462570.21851159891455507No Hit
GTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT1409210.21053948208043385No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1299800.1941933557157187No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACC1268580.18952900999680442No Hit
CTGAATCATACACATAAGACCAATATCTACTTTTGATCCAATATGAAGGC1234360.18441645680970495No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA1163490.17382830238627597No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA1014640.15158974183981905No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT1005920.15028695213229398No Hit
CCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCC982150.14673565495937305No Hit
GTTTACCTTAGTTCAATATCTACATCTTTAAGATCCAATATGAAGTTAGC946510.14141095023733258No Hit
CTTCTCTTTACATTCCAGTTTCACCTTGTTCGGTTTCTCCTTTCTGCATG917720.13710965256764837No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA917170.13702748119848107No Hit
CTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAG883020.13192538618563926No Hit
CTTTAGTACCTTTTGCATAAGGGTTTTTCAAGATCAATTTAAGAATTTAA849680.12694430718920743No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA822480.12288055947766141No Hit
CTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTAAATTTTT813310.12151053864990857No Hit
GTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACCATT807890.12070077715738727No Hit
ATTTTTTTGAGTTAAGAAATCGTGGTAGAGATTTGATTAAACTAAAGAAT804390.12017786844450451No Hit
GTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCT799790.11949061699328715No Hit
CTTCACCTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTT758580.1133337404053161No Hit
ATAAAAACGAGTAAAAATATTATTTTCTTGGTATCACTGTTTTGTTTTAC725280.1083586375084601No Hit
CCCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGC722890.10800156555880588No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA722270.10790893601538092No Hit
AATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAA718390.10732925435652804No Hit
CTTTAGTACCTTTTGCATAAGGGTTTTTCAAGATAAATTTAAGAATTTAA711150.1062475803333077No Hit
ATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCC680810.1017147088050611No Hit
TAGAAACCAAAGATTTTTTAAACATCAATTTCACCATTTTTCAGCTTAAC680230.10162805536121197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT107100.050.160861
CCCGTCA118400.041.529433
TGTGAAA2133600.037.056095
GTGCGCG22650.036.6891378
TTGTGAA2235650.035.5497634
CGCCTAT89700.035.342071
TGGCCCG37450.035.250128
GTGAAAA2314950.034.32556
GAAAAGT2487400.031.6065758
TTTGTGA2564300.031.052273
CGCGCTG8750.030.373021
TTTTGTG2720850.029.9825552
CGCCTAA75850.029.2192671
AGACGTG187850.028.9553474
CGATTAC159950.028.5163884
CGTGCAT180800.028.3335557
CCGTCAA174550.028.088974
CTGAATC457600.028.0017851
CATACAC446150.027.5717667
CTCCCAT842200.027.0591981
Produced by FastQC (version 0.11.2)