FastQCFastQC Report
Wed 25 Nov 2015
C0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66933289
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA3642630.5442179899451826No Hit
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA3195050.47734842374173486No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT2755450.4116710894036598No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC2417240.3611416734653514No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA2120090.3167467237416049No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA2118670.31653457220666387No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA2017100.3013597613588061No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1638540.24480195497340643No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT1543330.2305773439581013No Hit
GTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT1424470.2128193640686027No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACC1324350.19786118683036777No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1313300.19621028932255222No Hit
CTGAATCATACACATAAGACCAATATCTACTTTTGATCCAATATGAAGGC1267300.18933777481037875No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA1215110.18154045888884976No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT994820.14862858450000865No Hit
CCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCC967390.14453047421590176No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA925530.1382764860098239No Hit
CTTCTCTTTACATTCCAGTTTCACCTTGTTCGGTTTCTCCTTTCTGCATG889850.13294580518820762No Hit
GTTTACCTTAGTTCAATATCTACATCTTTAAGATCCAATATGAAGTTAGC888350.13272170145411502No Hit
ATAAAAACGAGTAAAAATATTATTTTCTTGGTATCACTGTTTTGTTTTAC835450.12481830976511553No Hit
CTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAG824650.12320476287964871No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA816250.12194978196873009No Hit
ATTTTTTTGAGTTAAGAAATCGTGGTAGAGATTTGATTAAACTAAAGAAT796080.11893633375763142No Hit
GTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACCATT779130.11640396156238489No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA776640.11603194936379117No Hit
GTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCT762720.11395226671141172No Hit
CTTTAGTACCTTTTGCATAAGGGTTTTTCAAGATCAATTTAAGAATTTAA761240.113731151027107No Hit
TAGAAACCAAAGATTTTTTAAACATCAATTTCACCATTTTTCAGCTTAAC733060.1095209888759538No Hit
AATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAA728790.10888304024623682No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA705710.1054348307909985No Hit
ATTTATTTAAAAATAAGCCTATGATCGGAGGGTCAGTGATTATGATAAAA701650.10482825668405447No Hit
ATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCC694010.10368682166507609No Hit
CTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTAAATTTTT692090.10339996888543755No Hit
CCCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGC670480.10017138108960999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT94850.045.758811
CCCGTCA106400.037.348133
CGCCTAT83300.037.0780531
TGGCCCG33750.036.0255368
GTGCGCG22300.035.5677078
TGTGAAA2090650.035.2713855
TTGTGAA2168950.033.0089074
GTGAAAA2256100.032.7689826
GAAAAGT2447400.030.0989328
CTGAATC446750.030.0827351
CATACAC460150.029.2512687
CGTGCAT179300.029.1642847
AGACGTG191500.028.9667724
CGGGCAT27100.028.2731251
TTTGTGA2457050.028.0632463
CGCCTAA72350.027.4292981
CGCGCTG10800.027.320281
TTTTGTG2612350.026.9444452
CGGAGTC77400.026.746411
TGTGCGC31850.026.3941757
Produced by FastQC (version 0.11.2)