Sampled 13 oysters for pre-stress epigenetic analyses.  In two plastic tanks filled with 6L, dissolved 300g of MgCl2 in each tank and left oysters in the solution overnight (3/11).  In the morning (3/12) sampled 13 oysters for DNA and RNA.  These tissues were put on dry ice and placed in the -80 freezer until further processing. 

Tomorrow will sample the additional 10 oysters left.  I decided to break the sampling up into two days since 23 oysters were too many to process at once.  After anesthetization the oysters only stay open for a minute or two so it would be too difficult to sample all oysters at once.  

Will hopefully do heat shock experiment later this week or early next week.

3/13/13
Sampled remaining 10 oysters for DNA and RNA tissues.  Placed oysters in net bags in Kristian's tanks until early next week when I will complete the heat shock experiment.  


Total number of oysters=23
Oyster samples that didn't open: oyster #1, 3, 19, 20
Total number of oysters sampled=19


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3/18/13
Sampled oysters that didn't open (#1, 3, 19, 20) in case we decide to do additional analyses on these samples.  Took DNA and RNA samples from oyster mantle and gill tissue and froze in -80 degree freezer
Measurements for oysters that didn't open:
1) 159.75g, 99cm L, 70.4cm W
3) 197.94g, 120.3cm L, 64.6cm W
19) 251.73g, 122.4cm L, 70.9cm W
20) 105.11g, 79.1cm L, 56.8cm W

3/19/13
Performed heat shock experiment.  Added about 8L of seawater to the water bath in the lab.  Put the remaining 19 oysters in the water bath at 40 degrees C for 1 hour.  Put 9 oysters back into aquarium room and will sample these in 1 week (3/26) and sampled 10 oysters immediately after the heat shock.  Took DNA and RNA samples from oyster mantle and gill tissue and froze in -80 degree freezer.
Measurements for first batch of oysters sampled:
2) 265.18g, 128.7cm L, 86.5cm W
4) 182.80g, 129.3 cm L, 73.5cm W
5) 127.82g, 99.3cm L, 64.3cm W
6) 186.19g, 115.5cm L, 92.9cm W
7) 209.47g, 134.8cm L, 68.6cm W
8) 49.73g, 71.4cm L, 45cm W
9) 233.55g, 126.1cm L, 69.3cm W
10) 120.05g, 99.7cm L, 57.1cm W
11) 185.84g, 116.3cm L, 72.2cm W
12) 190.16g, 116.9cm L, 69.8 cm W

3/26/13 sampled remaining heat shock oysters 1 week later
13) 123.19g, 98.5cm L, 56.1cm W
14) 371.16g, 133.2cm L, 92.5cm W
15) 184.46g, 107.5cm L, 61.8cm W
16) 191.0g, 112.7cm L, 67.3cm W
17) 80.19g, 76.6cm L, 56.2cm W
18) 147.27g, 109.5cm L, 71.6cm W
21) 236.64g, 120.5cm L, 70.5cm W
22) 144.13g, 112.5cm L, 67.1cm W
23)117.73g, 89.3cm L, 63.0cm W

**NOTE: 22+23- not sure which is which since labels came off!