Well / Set Group Efficiency target C(t) target expression Actin C(t) Actin expression relative expression A11 Neg control 66.83% 29.52 348.7996175 36.09 3.661777906 95.25417063 A12 Pos control 55.01% 34.39 11.90507128 18.51 722417.071 1.64795E-05 B11 Oyster 108.59% 23.7 19749.67712 19.9 275498.983 0.071686933 B12 Dabob 57.88% 32.88 33.92688604 29.05 483.2145469 0.070210813 C11 Oyster 95.86% 27.49 1425.646985 23.83 18046.95768 0.078996527 C12 Dabob 39.68% 34.79 9.020944274 24.03 15709.55923 0.000574233 D11 Oyster 105.80% 26.46 2912.379732 30.28 205.9036285 14.14438275 D12 Dabob 105.45% 24.86 8834.215721 28.24 847.4459334 10.42451839 E11 Oyster 138.86% 24.04 15600.98431 30.9 133.9429933 116.4748071 E12 Dabob 85.56% 26.39 3057.257366 29.44 368.6990342 8.292013491 F11 Oyster 131.16% 23.57 21613.04711 30.36 194.7906021 110.9552867 F12 Dabob 100.86% 25.33 6376.817677 22.05 62021.17646 0.10281678 G11 Neg control 69.58% 28.98 507.2522709 34.34 12.32514394 41.15589021 G12 Neg control 40.43% 32.74 37.38625811 33.88 16.95680399 2.204793907 H11 Neg control 22.65% 35.72 4.732994502 38.82 0.551335554 8.584598735 H12 Neg control 28.78% 33.7 19.21144359 N/A 0 0