FastQCFastQC Report
Wed 27 May 2015
filtered_108A_Male_Mix_AGTCAA_L007_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefiltered_108A_Male_Mix_AGTCAA_L007_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53400200
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT3369020.6309002588005289TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1114620.20872955532001752TruSeq Adapter, Index 2 (97% over 35bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCC585320.10961007636675517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA553350.019.9896581
CGGAAGA571400.019.7003544
AGATCGG180800.019.6316491
TCGGAAG566900.019.5583933
GCACACG572600.019.54822711
GAGCACA586300.019.2609529
AGAGCAC593000.019.0230968
ATCGGAA581700.019.0110912
TCACAGT571700.018.81294630
CACACGT604250.018.50445212
AGCACAC610450.018.46884310
ACACGTC606250.018.46072613
CACGTCT607050.018.41663414
GAAGAGC621450.018.16676
ACGTCTG624850.017.92800515
AAGAGCA641500.017.6199467
CGTCTGA637600.017.5765616
GAACTCC651050.017.185821
AGTCACA648450.016.72735228
CAGTCAC662450.016.64553327
Produced by FastQC (version 0.11.2)